Abstract
Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.
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References
Datta S, Malhotra L, Dickerson R, Chaffee S, Sen CK, Roy S (2015) Laser capture microdissection: Big data from small samples. Histol Histopathol 30:1255–1269
Hondius DC, Eigenhuis KN, Morrema THJ et al (2018) Proteomics analysis identifies new markers associated with capillary cerebral amyloid angiopathy in Alzheimer's disease. Acta Neuropathol Commun 6:46
Hondius DC, van Nierop P, Li KW, Hoozemans JJ, van der Schors RC, van Haastert ES, van der Vies SM, Rozemuller AJ, Smit AB (2016) Profiling the human hippocampal proteome at all pathologic stages of Alzheimer's disease. Alzheimers Dement 12:654–668
Drummond E, Nayak S, Faustin A et al (2017) Proteomic differences in amyloid plaques in rapidly progressive and sporadic Alzheimer's disease. Acta Neuropathol 133:933–954
Wong TH, Chiu WZ, Breedveld GJ et al (2014) PRKAR1B mutation associated with a new neurodegenerative disorder with unique pathology. Brain 137:1361–1373
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Hondius, D.C., Hoozemans, J.J.M., Rozemuller, A.J.M., Smit, A.B. (2019). Laser Microdissection for Spatial Proteomics of Postmortem Brain Tissue. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 146. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9662-9_4
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DOI: https://doi.org/10.1007/978-1-4939-9662-9_4
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9661-2
Online ISBN: 978-1-4939-9662-9
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