Abstract
To date, little is published on the characterization and therapeutic potential of human mesenchymal stromal cells (MSCs) derived from hair follicle dermal sheath (DS). We present protocols for the isolation and culture of human DS-MSCs starting with the use of a dissecting microscope to separate out dermal sheaths from hair follicles for trypsin digestion. We also present the protocols for the adipogenic, osteogenic, and chondrogenic differentiation of these DS-MSCs as we seek to harness these cells for potential applications in stem cell therapy and tissue engineering.
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References
Richardson GD, Arnott EC, Whitehouse CJ, Lawrence CM, Reynolds AJ, Hole N et al (2005) Plasticity of rodent and human hair follicle dermal cells: implications for cell therapy and tissue engineering. J Investig Dermatol Symp Proc 10:180–183
Ma D, Kua JE, Lim WK, Lee ST, Chua AW (2015) In vitro characterization of human hair follicle dermal sheath mesenchymal stromal cells and their potential in enhancing diabetic wound healing. Cytotherapy 17:1036–1051
Richardson GD, Arnott EC, Whitehouse CJ, Lawrence CM, Hole N, Jahoda CA (2005) Cultured cells from the adult human hair follicle dermis can be directed toward adipogenic and osteogenic differentiation. J Invest Dermatol 124:1090–1091
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Ma, D., Lee, ST., Chua, A.W.C. (2019). Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells. In: Böttcher-Haberzeth, S., Biedermann, T. (eds) Skin Tissue Engineering. Methods in Molecular Biology, vol 1993. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9473-1_5
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DOI: https://doi.org/10.1007/978-1-4939-9473-1_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9472-4
Online ISBN: 978-1-4939-9473-1
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