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Titration of Mass Cytometry Reagents

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Mass Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1989))

Abstract

Mass cytometry (MC) is a powerful research tool enabling high-dimensional analysis of single cells in suspension and within tissue sections following laser ablation. Here we describe the procedure of titrating metal-conjugated antibodies, to ensure that optimal levels of staining are achieved while minimizing nonspecific signals that may occur at high concentrations.

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References

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Author information

Authors and Affiliations

  1. Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia

    Caryn van Vreden

  2. Ramaciotti Facility for Human Systems Biology, The University of Sydney, Sydney, Australia

    Caryn van Vreden & Barbara Fazekas de St. Groth

  3. Charles Perkins Centre, The University of Sydney, Sydney, Australia

    Caryn van Vreden, Paula Niewold & Barbara Fazekas de St. Groth

  4. Discipline of Pathology, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia

    Paula Niewold & Barbara Fazekas de St. Groth

  5. Ramaciotti Facility for Human Systems Biology, The University of Sydney, Sydney, NSW, Australia

    Helen M. McGuire

  6. Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia

    Helen M. McGuire

  7. Discipline of Pathology, The University of Sydney, Camperdown, NSW, Australia

    Helen M. McGuire

Authors
  1. Caryn van Vreden
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  2. Paula Niewold
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  3. Helen M. McGuire
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  4. Barbara Fazekas de St. Groth
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Corresponding author

Correspondence to Barbara Fazekas de St. Groth .

Editor information

Editors and Affiliations

  1. Discipline of Pathology, The University of Sydney, Camperdown, NSW, Australia

    Helen M. McGuire

  2. Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, NSW, Australia

    Thomas M. Ashhurst

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Cite this protocol

van Vreden, C., Niewold, P., McGuire, H.M., Fazekas de St. Groth, B. (2019). Titration of Mass Cytometry Reagents. In: McGuire, H., Ashhurst, T. (eds) Mass Cytometry. Methods in Molecular Biology, vol 1989. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9454-0_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9454-0_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9453-3

  • Online ISBN: 978-1-4939-9454-0

  • eBook Packages: Springer Protocols

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