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Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays

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Neisseria meningitidis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1969))

Abstract

Serum bactericidal antibody (SBA) assays measure functional antibody titers against Neisseria meningitidis in sera. Induction of complement-dependent SBA after vaccination with meningococcal polysaccharide or conjugate or protein based vaccines is regarded as the surrogate of protection and thus acceptable evidence of the potential efficacy of these vaccines. This chapter discusses and details SBA assay protocols for measuring the complement-mediated lysis of serogroup A, B, C, W, X, and Y meningococci by human sera, for example, following vaccination or disease.

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Authors and Affiliations

  1. Meningococcal Reference Unit, National Infection Service, Public Health England, Manchester Medical Microbiology Partnership, Manchester Royal Infirmary, Manchester, UK

    Jay Lucidarme & Ray Borrow

  2. Vaccine Evaluation Unit, National Infection Service, Public Health England, Manchester Medical Microbiology Partnership, Manchester Royal Infirmary, Manchester, UK

    Jennifer Louth, Kelly Townsend-Payne & Ray Borrow

Authors
  1. Jay Lucidarme
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  2. Jennifer Louth
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  3. Kelly Townsend-Payne
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  4. Ray Borrow
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Corresponding author

Correspondence to Ray Borrow .

Editor information

Editors and Affiliations

  1. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Kate L. Seib

  2. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Ian R. Peak

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Lucidarme, J., Louth, J., Townsend-Payne, K., Borrow, R. (2019). Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays. In: Seib, K., Peak, I. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 1969. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9202-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9202-7_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9201-0

  • Online ISBN: 978-1-4939-9202-7

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