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Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells

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  • First Online:
Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1922))

Abstract

This protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars and for the generation of high-titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequent experiments.

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References

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Author information

Authors and Affiliations

  1. Department of Oral Biology, College of Dentistry, The University of Illinois at Chicago, Chicago, IL, USA

    Elizabeth Guirado, Youbin Zhang & Anne George

Authors
  1. Elizabeth Guirado
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  2. Youbin Zhang
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  3. Anne George
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Corresponding author

Correspondence to Anne George .

Editor information

Editors and Affiliations

  1. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

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Cite this protocol

Guirado, E., Zhang, Y., George, A. (2019). Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9012-2_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9011-5

  • Online ISBN: 978-1-4939-9012-2

  • eBook Packages: Springer Protocols

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