Abstract
In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.
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Wu, J., Feng, J.Q., Wang, X. (2019). In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_16
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DOI: https://doi.org/10.1007/978-1-4939-9012-2_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9011-5
Online ISBN: 978-1-4939-9012-2
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