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Reactive Oxygen Species Detection in Senescent Cells

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Cellular Senescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1896))

Abstract

Cumulative evidence suggests that cellular senescence plays a variety of important physiological roles, including tumor suppression, embryonic development and ageing. Senescent cells are characterized by increased production of reactive oxygen species (ROS), mostly produced by dysfunctional mitochondria. Both intracellular and extracellular ROS have been shown to contribute to the induction of senescence. ROS have also been shown to act as signaling molecules during senescence, stabilizing the cell-cycle arrest. In this chapter, we present a detailed description of protocols that allow us to characterize intracellular and extracellular ROS in live senescent cells.

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Author information

Authors and Affiliations

  1. Newcastle University Institute for Ageing, Newcastle University, Newcastle upon Tyne, UK

    Stella Victorelli & João F. Passos

  2. Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK

    Stella Victorelli & João F. Passos

  3. Ageing Research Laboratories, Campus for Ageing and Vitality, Newcastle University, Newcastle upon Tyne, UK

    Stella Victorelli & João F. Passos

Authors
  1. Stella Victorelli
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  2. João F. Passos
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Corresponding author

Correspondence to João F. Passos .

Editor information

Editors and Affiliations

  1. ERIBA, University Medical Center Groningen, Groningen, Groningen, The Netherlands

    Marco Demaria

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Victorelli, S., Passos, J.F. (2019). Reactive Oxygen Species Detection in Senescent Cells. In: Demaria, M. (eds) Cellular Senescence. Methods in Molecular Biology, vol 1896. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8931-7_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8931-7_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8930-0

  • Online ISBN: 978-1-4939-8931-7

  • eBook Packages: Springer Protocols

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