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Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells

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The Hippo Pathway

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

The transcriptional coactivator with PDZ-binding motif (TAZ), which is encoded by the WWTR1 gene, is a key transcriptional effector of the Hippo signaling pathway. TAZ function has been implicated in a variety of developmental processes and diseases, most notably in driving oncogenesis. Given that nuclear-cytoplasmic localization dynamics dictate TAZ activity, techniques for visualizing TAZ localization are critical for its study. Here we describe an immunofluorescence microscopy protocol that allows for the visualization of TAZ subcellular localization in mammalian cells, offering an approach that can aid in the analysis of TAZ regulation and function.

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Acknowledgments

We would like to thank Dr. Jeffrey Wrana (Lunenfeld-Tanenbaum Research Institute, Toronto, Canada) for providing the Taz-loxP/loxP mice. X.V. is supported by NIH R01HL124392, the March of Dimes Foundation Grant no. 1-FY17-375, and an American Cancer Society Ellison New England Research Scholar Grant (RSG-17-138-01-CSM). N. M. K. is supported by NIH T32HL007035-40. J.H.B. is supported by NIH F31HL13250601.

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Authors and Affiliations

  1. Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA

    Nathan M. Kingston, Andrew M. Tilston-Lunel, Julia Hicks-Berthet & Xaralabos Varelas

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  1. Nathan M. Kingston
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  2. Andrew M. Tilston-Lunel
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  3. Julia Hicks-Berthet
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  4. Xaralabos Varelas
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Corresponding author

Correspondence to Xaralabos Varelas .

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Editors and Affiliations

  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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Kingston, N.M., Tilston-Lunel, A.M., Hicks-Berthet, J., Varelas, X. (2019). Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

  • eBook Packages: Springer Protocols

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