Abstract
The tumor microenvironment plays a vital role in therapeutic resistance in pancreatic adenocarcinoma. We have created a 3D model to study the tumor microenvironment ex vivo that integrates tumor cells with tumor associated macrophages (TAMs) and cancer associated fibroblasts (CAFs). The microtumor model recreates the complex histology of a primary tumor and can be analyzed histologically or with viability assays for therapeutic testing. Microtumors demonstrate increased resistance to gemcitabine when compared to tumor cells alone in a 3D spheroid, highlighting the contribution of TAMs and CAFs when designing models for therapeutic testing.
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Goodwin, M.L., Urs, S.K., Simeone, D.M. (2019). Pancreatic Microtumors: A Novel 3D Ex Vivo Testing Platform. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_7
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DOI: https://doi.org/10.1007/978-1-4939-8879-2_7
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8879-2
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