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Tricine-SDS-PAGE

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Electrophoretic Separation of Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1855))

Abstract

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) is an efficient way of separating low molecular mass proteins. However, the standard system is quite complicated and specifically may not be useful when the separated proteins are to be recovered from the gel for quantitative analysis. Here, we describe a simplified system whereby these smaller proteins can be resolved in comparatively low percentage gels which have high compatibility with modern detectors such as UV and inductively coupled plasma-mass spectrometry (ICP-MS).

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Acknowledgments

This work was supported by a grant from Loughborough University, Loughborough, Leicestershire, LE11 3TU, UK.

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Authors and Affiliations

  1. Department of Chemistry, Centre for Analytical Science, Loughborough University, Loughborough, UK

    Syed R. Haider, Helen J. Reid & Barry L. Sharp

Authors
  1. Syed R. Haider
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  2. Helen J. Reid
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  3. Barry L. Sharp
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Corresponding author

Correspondence to Barry L. Sharp .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Biji T. Kurien

  2. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    R. Hal Scofield

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Haider, S.R., Reid, H.J., Sharp, B.L. (2019). Tricine-SDS-PAGE. In: Kurien, B., Scofield, R. (eds) Electrophoretic Separation of Proteins. Methods in Molecular Biology, vol 1855. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8793-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8793-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8792-4

  • Online ISBN: 978-1-4939-8793-1

  • eBook Packages: Springer Protocols

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