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How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique

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Xenopus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1865))

Abstract

We have taken advantage of the well-established oocyte host transfer technique to optimize a method for CRISPR editing of Xenopus that provides an efficient non-mosaic targeted insertion of small DNA fragment through homology-directed repair mechanism.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, Alabama State University, Montgomery, AL, USA

    Emmanuel Tadjuidje

  2. Cincinnati Children’s Research Foundation, Cincinnati, OH, USA

    Sang-Wook Cha

  3. Department of Biology and Agriculture, School of Natural Sciences, University of Central Missouri, Warrensburg, MO, USA

    Sang-Wook Cha

Authors
  1. Emmanuel Tadjuidje
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  2. Sang-Wook Cha
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Corresponding author

Correspondence to Sang-Wook Cha .

Editor information

Editors and Affiliations

  1. Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium

    Kris Vleminckx

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Tadjuidje, E., Cha, SW. (2018). How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique. In: Vleminckx, K. (eds) Xenopus. Methods in Molecular Biology, vol 1865. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8784-9_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8784-9_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8783-2

  • Online ISBN: 978-1-4939-8784-9

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