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Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin

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Xenopus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1865))

Abstract

Analyses by flow cytometry and differential counts by cytospin of peripheral blood leukocytes are two reliable and convenient methods used to assess the immune status and immune responses in the amphibian Xenopus. Here, we describe these methods and discuss their challenges and limitations.

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References

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Acknowledgments

The authors are supported by R24-AI-059830 and IOB-074271 from the NSF.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA

    Jacques Robert, Eva-Stina Edholm & Francisco De Jesus Andino

  2. The Norwegian College of Fishery Science, University of Tromsø, Tromsø, Norway

    Eva-Stina Edholm

Authors
  1. Jacques Robert
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  2. Eva-Stina Edholm
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  3. Francisco De Jesus Andino
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Corresponding author

Correspondence to Jacques Robert .

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Editors and Affiliations

  1. Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium

    Kris Vleminckx

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Robert, J., Edholm, ES., De Jesus Andino, F. (2018). Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin. In: Vleminckx, K. (eds) Xenopus. Methods in Molecular Biology, vol 1865. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8784-9_19

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  • DOI: https://doi.org/10.1007/978-1-4939-8784-9_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8783-2

  • Online ISBN: 978-1-4939-8784-9

  • eBook Packages: Springer Protocols

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