Abstract
Many developmental signals are associated with changes in proliferative response. Also, growing organs and tissues can contain different cellular subpopulations with a defined status in the cell cycle, e.g., quiescent in stem cells, high proliferation in progenitors, cell cycle exit in differentiating cells. This chapter describes a method for isolation of individual cell populations from the Xenopus tadpole brain and determination of their cell cycle status using flow cytometry.
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Acknowledgments
The authors would like to thank Dr. Muriel Perron for sharing the protocol on which ours is based. Research in the authors’ laboratory is supported by the Research Foundation—Flanders (FWO-Vlaanderen) (grants G0A1515N and G029413N), by the Belgian Science Policy (Interuniversity Attraction Poles—IAP7/07) and by the Concerted Research Actions from Ghent University (BOF15/GOA/011). Further support was obtained by the Hercules Foundation, Flanders (grant AUGE/11/14) and the Desmoid Tumor Research Foundation.
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Noelanders, R., Vleminckx, K. (2018). Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry. In: Vleminckx, K. (eds) Xenopus. Methods in Molecular Biology, vol 1865. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8784-9_17
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DOI: https://doi.org/10.1007/978-1-4939-8784-9_17
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