Abstract
Dual modality reporter genes are powerful means of tracking cellular processes in cell culture systems and whole animals. In this chapter, we describe the methods for construction of a plasmid reporter gene vector expressing a fluorescent and a bioluminescent gene and its validation by in vitro assays in mammalian cells as well as by noninvasive imaging methods in small animal models.
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Dhadve, A., Thakur, B., Ray, P. (2018). Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging. In: Dubey, P. (eds) Reporter Gene Imaging. Methods in Molecular Biology, vol 1790. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7860-1_2
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DOI: https://doi.org/10.1007/978-1-4939-7860-1_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7858-8
Online ISBN: 978-1-4939-7860-1
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