Abstract
The availability of more specific dyes for a subset of endomembrane compartments, combined with the development of genetically encoded probes and advanced microscopy technologies, makes live cell imaging an approach that goes beyond the microscopically observation of cell structure. Here we describe the latest improved techniques to investigate protein–protein interaction, protein topology, and protein dynamics.
Furthermore, we depict new technical approaches to identify mutants for chloroplast morphology and distribution through the tracking of chlorophyll fluorescence, as well as mutants for chloroplast movement.
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Renna L, Stefano G, Majeran W, Micalella C, Meinnel T, Giglione C, Brandizzi F (2013) Plant Cell 25:1756–1773
Stefano G, Renna L, Rossi M, Azzarello E, Pollastri S, Brandizzi F, Baluska F, Mancuso S (2010) Plant J 64:790–799
Takagi J, Renna L, Takahashi H, Koumoto Y, Tamura K, Stefano G, Fukao Y, Kondo M, Nishimura M, Shimada T, Brandizzi F, Hara-Nishimura I (2013) Plant Cell 25:4658–4675
Higuchi R, Krummel B, Saiki RK (1988) Nucleic Acids Res 16:7351–7367
Kohler R, Hanson M, Wildman S (1997) Trends Cell Biol 7:392–392
Larkin RM, Stefano G, Ruckle ME, Stavoe AK, Sinkler CA, Brandizzi F, Malmstrom CM, Osteryoung KW (2016) Proc Natl Acad Sci U S A 113:E1116–E1125
Berendzen KW, Bohmer M, Wallmeroth N, Peter S, Vesic M, Zhou Y, Tiesler FK, Schleifenbaum F, Harter K (2012) Plant Methods 8:25
Miller KE, Kim Y, Huh WK, Park HO (2015) J Mol Biol 427:2039–2055
Lalonde S, Ehrhardt DW, Loque D, Chen J, Rhee SY, Frommer WB (2008) Plant J 53:610–635
Kodama Y, Hu CD (2012) BioTechniques 53:285–298
Stefano G, Renna L, Brandizzi F (2015) Methods Mol Biol 1242:173–182
Grefen C, Blatt MR (2012) BioTechniques 53:311–314
Gookin TE, Assmann SM (2014) Plant J 80:553–567
Wan Y, Ash WM 3rd, Fan L, Hao H, Kim MK, Lin J (2011) Plant Methods 7:27
Anderson CT, Carroll A, Akhmetova L, Somerville C (2010) Plant Physiol 152:787–796
Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR (1989) Gene 77:51–59
Clough SJ, Bent AF (1998) Plant J 16:735–743
Brandizzi F, Snapp EL, Roberts AG, Lippincott-Schwartz J, Hawes C (2002) Plant Cell 14:1293–1309
Stefano G, Renna L, Brandizzi F (2014) J Cell Sci 127:947–953
Stefano G, Osterrieder A, Hawes C, Brandizzi F (2013) Methods Cell Biol 118:69–83
Serodio J, Ezequiel J, Frommlet J, Laviale M, Lavaud J (2013) Plant Physiol 163:1089–1102
Muller SM, Galliardt H, Schneider J, Barisas BG, Seidel T (2013) Front Plant Sci 4:413
Karperien A., http://rsb.info.nih.gov/ij/plugins/fraclac/FLHelp/Introduction.htm., (1999-2013)
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Renna, L., Stefano, G., Brandizzi, F. (2018). Live Cell Imaging and Confocal Microscopy. In: Pereira, C. (eds) Plant Vacuolar Trafficking. Methods in Molecular Biology, vol 1789. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7856-4_9
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DOI: https://doi.org/10.1007/978-1-4939-7856-4_9
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