Abstract
Zebrafish has emerged as an informative animal model to study the biological impact and molecular mechanisms of hypoxia. Here we describe a simple method to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and can be applied to other fish and amphibian species.
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Acknowledgments
This work was supported by Grant-in-Aid for Young Scientists [(B)# 15K18799] to HK from JSPS and by National Science Foundation grant IOS-1557850 and University of Michigan MCube2.0 Project U0496246 to CD.
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Kamei, H., Duan, C. (2018). Hypoxic Treatment of Zebrafish Embryos and Larvae. In: Huang, L. (eds) Hypoxia. Methods in Molecular Biology, vol 1742. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7665-2_17
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DOI: https://doi.org/10.1007/978-1-4939-7665-2_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7664-5
Online ISBN: 978-1-4939-7665-2
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