Abstract
Traumatic brain injury (TBI) diagnoses have increased in frequency during the past decade, becoming a silent epidemic. The pathophysiology of TBI involves pathophysiological processes affecting the brain, induced by traumatic biomechanical forces resulting in temporary impairment of neurological function. Preclinical models have been generated to recapitulate the mechanical, neuroinflammatory, and behavioral outcomes observed in the clinical setting. The lateral fluid percussion (LFP) model is the most extensively used and well-characterized model of nonpenetrating and nonischemic TBI. The model is reproducible and can be adjusted to produce a mild to moderate and severe injury, as reflected by mortality and return of reflexes, by adjusting the amount of force applied. The histopathological changes achieved with this model reproduce that seen in human TBI including focal contusion in the cortex, with accompanying intraparenchymal punctate hemorrhage, followed by inflammation and neuronal degeneration. This chapter describes the LFP model, which produces a mixed model of focal and diffuse brain injury that progresses over time affecting predominantly the cortical parenchyma.
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References
Faul M, Xu L, Wald M, Coronado V (2010) Traumatic brain injury in the United States, Emergency Department visits, hospitalizations and deaths 2002–2006. Center for Disease Control and Prevention, National Center for Injury Prevention and Control, Atlanta, GA
Feinstein A, Rapoport M (2000) Mild traumatic brain injury: the silent epidemic. Can J Public Health 91:325–326. 32
Pellman EJ, Powell JW, Viano DC, Casson IR, Tucker AM, Feuer H, Lovell M, Waeckerle JF, Robertson DW (2004) Concussion in professional football, epidemiological features of game injuries and review of the literature—part 3. Neurosurgery 54:81–94. discussion -6
Werner C, Engelhard K (2007) Pathophysiology of traumatic brain injury. Br J Anaesth 99:4–9
Konrad C, Geburek AJ, Rist F, Blumenroth H, Fischer B, Husstedt I, Arolt V, Schiffbauer H, Lohmann H (2011) Long-term cognitive and emotional consequences of mild traumatic brain injury. Psychol Med 41(6):1197–1211
Gentleman SM, Leclercq PD, Moyes L, Graham DI, Smith C, Griffin WS, Nicoll JA (2004) Long-term intracerebral inflammatory response after traumatic brain injury. Forensic Sci Int 146:97–104
Riggio S (2011) Traumatic brain injury and its neurobehavioral sequelae. Neurol Clin 29:35–47
Dixon CE, Lyeth BG, Povlishock JT, Findling RL, Hamm RJ, Marmarou A, Young HF, Hayes RL (1987) A fluid percussion model of experimental brain injury in the rat. J Neurosurg 67:110–119
McIntosh TK, Vink R, Noble L, Yamakami I, Fernyak S, Soares H, Faden AL (1989) Traumatic brain injury in the rat, characterization of a lateral fluid-percussion model. Neuroscience 28:233–244
Dixon CE, Clifton GL, Lighthall JW, Yaghmai AA, Hayes RL (1991) A controlled cortical impact model of traumatic brain injury in the rat. J Neurosci Methods 39:253–262
Marmarou A, Foda MA, van den Brink W, Campbell J, Kita H, Demetriadou K (1994) A new model of diffuse brain injury in rats. Part I, pathophysiology and biomechanics. J Neurosurg 80:291–300
Thompson HJ, Lifshitz J, Marklund N, Grady MS, Graham DI, Hovda DA, McIntosh TK (2005) Lateral fluid percussion brain injury, a 15-year review and evaluation. J Neurotrauma 22:42–75
Povlishock JT (1993) Pathobiology of traumatically induced axonal injury in animals and man. Ann Emerg Med 22(6):980
Ling GSF, Lee EY, Kalehua AN (2001) Traumatic brain injury in the rat using the fluid-percussion model. Current protocols in neuroscience. Wiley, New York
Acknowledgments
We would like to acknowledge Jesse Sulzer, MD, PhD; Sophie Teng, MD, PhD; Renata Impastato; Emily Rogers; and Kylie Mills who have contributed to the refinement of this model.
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Katz, P.S., Molina, P.E. (2018). A Lateral Fluid Percussion Injury Model for Studying Traumatic Brain Injury in Rats. In: Tharakan, B. (eds) Traumatic and Ischemic Injury. Methods in Molecular Biology, vol 1717. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7526-6_3
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DOI: https://doi.org/10.1007/978-1-4939-7526-6_3
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