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A Murine Model of Myocardial Ischemia–Reperfusion Injury

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Traumatic and Ischemic Injury

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1717))

Abstract

Ligation of the left anterior descending (LAD) coronary artery in the mouse heart is a widely used model to simulate myocardial infarction and ischemia–reperfusion injury. Here we describe a ligation technique routinely performed in our laboratory to induce myocardial infarction that may be used to study ischemia–reperfusion injury in the myocardium. The methods described enhance location of the LAD coronary artery to allow for accurate ligation, thus increasing reproducibility of infarct size and location.

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Author information

Author notes

  1. Zhaobin Xu and Kevin E. McElhanon contributed equally to this work.

Authors and Affiliations

  1. Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute, Ohio State University Wexner Medical Center, Columbus, OH, USA

    Zhaobin Xu, Kevin E. McElhanon, Eric X. Beck & Noah Weisleder

Authors
  1. Zhaobin Xu
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  2. Kevin E. McElhanon
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  3. Eric X. Beck
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  4. Noah Weisleder
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Corresponding author

Correspondence to Noah Weisleder .

Editor information

Editors and Affiliations

  1. Department of Surgery, Texas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research Institute, Temple, Texas, USA

    Binu Tharakan

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Xu, Z., McElhanon, K.E., Beck, E.X., Weisleder, N. (2018). A Murine Model of Myocardial Ischemia–Reperfusion Injury. In: Tharakan, B. (eds) Traumatic and Ischemic Injury. Methods in Molecular Biology, vol 1717. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7526-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7526-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7524-2

  • Online ISBN: 978-1-4939-7526-6

  • eBook Packages: Springer Protocols

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