Abstract
Chaperones associate with hundreds or thousands of diverse client proteins and regulate their function. Chaperone/client interactions are generally very transient and involve a highly orchestrated assembly and disassembly of regulatory co-factors. This poses specific challenges for identifying and characterizing these interactions in a scalable and sensitive manner. LUMIER assay, which takes advantage of the high sensitivity and linear range of luminescence-based detection, has proven to be an ideal assay to quantitatively profile chaperone/client interactions in a high-throughput manner. This article provides step-by-step instructions for quantitatively profiling these interactions with LUMIER.
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Taipale, M. (2018). Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay. In: Calderwood, S., Prince, T. (eds) Chaperones. Methods in Molecular Biology, vol 1709. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7477-1_4
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DOI: https://doi.org/10.1007/978-1-4939-7477-1_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7477-1
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