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Nucleotide Exchange Factors for Hsp70 Chaperones

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Chaperones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1709))

Abstract

The ATPase cycle of Hsp70 chaperones controls their transient association with substrates and thus governs their function in protein folding. Nucleotide exchange factors (NEFs) accelerate ADP release from Hsp70, which results in rebinding of ATP and release of the substrate, thereby regulating the lifetime of the Hsp70-substrate complex. This chapter describes several methods suitable to study NEFs of Hsp70 chaperones. On the one hand, steady-state ATPase assays provide information on how the NEF influences progression of the Hsp70 through the entire ATPase cycle. On the other hand, nucleotide release can be measured directly using labeled nucleotides, which enables identification and further characterization of NEFs.

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Author information

Authors and Affiliations

  1. Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany

    Heike Rampelt, Matthias P. Mayer & Bernd Bukau

  2. Faculty of Medicine, Institute of Biochemistry and Molecular Biology, ZMBZ, 79104, Freiburg, Germany

    Heike Rampelt

Authors
  1. Heike Rampelt
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  2. Matthias P. Mayer
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  3. Bernd Bukau
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Corresponding author

Correspondence to Matthias P. Mayer .

Editor information

Editors and Affiliations

  1. Department of Radiation, Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA

    Stuart K. Calderwood

  2. Department of Urology, Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania, USA

    Thomas L. Prince

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Rampelt, H., Mayer, M.P., Bukau, B. (2018). Nucleotide Exchange Factors for Hsp70 Chaperones. In: Calderwood, S., Prince, T. (eds) Chaperones. Methods in Molecular Biology, vol 1709. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7477-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7477-1_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7476-4

  • Online ISBN: 978-1-4939-7477-1

  • eBook Packages: Springer Protocols

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