Abstract
Photorespiratory metabolism is compartmented over the chloroplast, peroxisome, cytosol, and mitochondria, and due to its complex structure it is often the case that metabolite levels alone are not able to fully describe photorespiration. Metabolic fluxes represent a more meaningful biological description of metabolism, adding to metabolite levels and often revealing different aspects of the system such as the presence of inactive metabolic pools of photorespiratory intermediates. We describe here a protocol for the 13CO2 feeding of Arabidopsis and tracing of 13C enriched metabolites for metabolic fluxes estimation, which allows high throughput analysis of labeling pattern on different metabolites involved in photorespiration and downstream processes.
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Acknowledgement
This work was financially supported by the Max Planck Society. We thank National Council for Scientific and Technological Development CNPq-Brazil for financially supporting LPS.
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de Souza, L.P., Szecówka, M., Fernie, A.R., Tohge, T. (2017). 13CO2 Labeling and Mass Spectral Analysis of Photorespiration. In: Fernie, A., Bauwe, H., Weber, A. (eds) Photorespiration. Methods in Molecular Biology, vol 1653. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7225-8_11
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DOI: https://doi.org/10.1007/978-1-4939-7225-8_11
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