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CRISPR/Cas9 in the Chicken Embryo

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Avian and Reptilian Developmental Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1650))

Abstract

Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.

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Acknowledgments

We thank Dr. Daniel Sieiro for critical reading of the manuscript. This work was supported by grants from the National Health and Medical Research Council (NHMRC, Australia) to C.M. and N.V. and by the Programme Avenir Lyon Saint-Etienne (PALSE) from the University of Lyon to C.M and V.M. The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government. The NeuroMyoGene Institute is supported by grants from the Association Française contre les Myopathies (AFM).

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Authors and Affiliations

  1. Institut NeuroMyoGène, INMG, Faculty of Medicine Laënnec, University Lyon1, Bâtiment B, 7 rue Guillaume Paradin, 69008, Lyon, France

    Valérie Morin & Christophe Marcelle

  2. EMBL Australia, Australian Regenerative Medicine Institute, Monash University, 15 Innovation Walk, Clayton, VIC, 3800, Australia

    Nadège Véron & Christophe Marcelle

Authors
  1. Valérie Morin
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  2. Nadège Véron
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  3. Christophe Marcelle
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Corresponding author

Correspondence to Christophe Marcelle .

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Editors and Affiliations

  1. IRCMS, Kumamoto University, Kumamoto, Kumamoto, Japan

    Guojun Sheng

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Morin, V., Véron, N., Marcelle, C. (2017). CRISPR/Cas9 in the Chicken Embryo. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7216-6_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7215-9

  • Online ISBN: 978-1-4939-7216-6

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