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In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells

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Avian and Reptilian Developmental Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1650))

Abstract

Reptiles and birds have been highlighted as excellent experimental models for the study of developmental biology; however, due to technical limitations in cellular analysis, dynamics of neural stem/progenitor cells of these animals remain unclear. In this chapter, we introduce the protocols for neurosphere culture and ex ovo embryonic culture of developing reptilian and avian embryos, which are modified from the method originally established for rodent embryos. Applications of these techniques provide powerful strategies for the study of comparative neural development of amniotes.

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Acknowledgments

We thank Ms. Misato Kawami for technical assistance, Dr. Masanori Uchikawa for pCAGGS-mRFP vector, and all members of Prof. Ono lab for valuable discussion. This work is supported by the fellowship of Japan Society for the Promotion of Science (JSPS) to WY, KAKENHI (The Empathetic Systems, #26118510, Thermal biology, #16H01394) to TN.

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Authors and Affiliations

  1. Developmental Neurobiology, Kyoto Prefectural University of Medicine, INAMORI Memorial Building, 1-5 Shimogamo-hangi cho, Sakyoku, Kyoto, 606-0823, Japan

    Wataru Yamashita, Toyo Shimizu & Tadashi Nomura

Authors
  1. Wataru Yamashita
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  2. Toyo Shimizu
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  3. Tadashi Nomura
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Corresponding author

Correspondence to Tadashi Nomura .

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Editors and Affiliations

  1. IRCMS, Kumamoto University, Kumamoto, Kumamoto, Japan

    Guojun Sheng

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Yamashita, W., Shimizu, T., Nomura, T. (2017). In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7216-6_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7215-9

  • Online ISBN: 978-1-4939-7216-6

  • eBook Packages: Springer Protocols

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