Skip to main content
SpringerLink
Log in

Handling of Gametes for In Vitro Insemination in Birds

  • Protocol
  • First Online:
Avian and Reptilian Developmental Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1650))

Abstract

A characteristic biological property of avian gamete (e.g., extremely large egg and polyspermic fertilization) does not allow the direct observation of sperm-egg interactions in vitro, but recent research advances make it possible to manipulate the gamete in vitro. Here, we describe the techniques for the handling of gametes required for in vitro fertilization assay. In addition, we also introduce the procedures for sperm-perivitelline membrane assay, intracytoplasmic sperm injection, and ex ovo culture.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Birkhead TR, Sheldon BC, Fletcher F (1994) A comparative study of sperm-egg interaction in birds. J Reprod Fertil 101:353–361

    Article  CAS  PubMed  Google Scholar 

  2. Kuroki M, Mori M (1997) Binding of spermatozoa to the perivitelline layer in the presence of a protease inhibitor. Poult Sci 76:748–752

    Article  CAS  PubMed  Google Scholar 

  3. Robertson L, Brown HL, Staines HJ, Wishart GJ (1997) Characterization and application of an avian in vitro spermatozoa-egg interaction assay using the inner perivitelline layer from laid chicken eggs. J Reprod Fertil 110:205–211

    Article  CAS  PubMed  Google Scholar 

  4. Robertson L, Wilson YI, Lindsay C, Wishart GJ (1998) Evaluation of semen from individual male domestic fowl by assessment of sperm: perivitelline interaction in vitro and in vivo. Bri Pout Sci 39:278–281

    Article  CAS  Google Scholar 

  5. Wishart GJ (1997) Quantitative aspects of sperm:egg interaction in chickens and turkeys. Anim Reprod Sci 48:81–92

    Article  CAS  PubMed  Google Scholar 

  6. Yanagimachi R (2005) Intracytoplasmic injection of spermatozoa and spermatogenic cells:its biology and applications in humans and animals. Reprod Biomed Online 10:247–288

    Article  PubMed  Google Scholar 

  7. Morozumi K, Shikano T, Miyazaki S, Yanagimachi R (2006) Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development. Proc Natl Acad Sci U S A 103:17661–17666

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Iwao Y (2012) Egg activation in physiological polyspermy. Reproduction 144:11–22

    Article  CAS  PubMed  Google Scholar 

  9. Hrabia A, Takagi S, Ono T, Shimada K (2003) Fertilization and development of quail oocytes after intracytoplasmic sperm injection. Biol Reprod 69:1651–1657

    Article  CAS  PubMed  Google Scholar 

  10. Mizushima S, Takagi S, Ono T, Atsumi Y, Tsukada A, Saito N, Shimada K (2009) Phospholipase Cζ mRNA expression and its potency during spermatogenesis for activation of quail oocyte as a sperm factor. Mol Reprod Dev 76:1200–1207

    Article  CAS  PubMed  Google Scholar 

  11. Mizushima S, Takagi S, Ono T, Atsumi Y, Tsukada A, Saito N, Sasanami T, Okabe M, Shimada K (2010) Novel method of gene transfer in birds: intracytoplasmic sperm injection for green fluorescent protein expression in quail blastoderms. Biol Reprod 83:965–969

    Article  CAS  PubMed  Google Scholar 

  12. Mizushima S, Hiyama G, Shiba K, Inaba K, Dohra H, Ono T, Shimada K, Sasanami T (2014) The birth of quail chicks after intracytoplasmic sperm injection. Development 141:3799–3806

    Article  CAS  PubMed  Google Scholar 

  13. Ono T (2001) Ex ovo culture of quail embryos and its application for embryo manipulation. Anim Sci J 72:361–371

    Google Scholar 

  14. Kato A, Mizushima S, Shimada K, Kagami H, Ono T (2014) Simple culture system for bobwhite quail and Japanese quail embryos from the blastoderm stage to hatching using a single surrogate eggshell. J Poult Sci 51:203–206

    Google Scholar 

  15. Woodard AE, Mather FB (1964) The timing of ovulation, movement of the ovum through the oviduct, pigmentation and shell deposition in Japanese quail (Coturnix Coturnix japonica). Poult Sci 43:1427–1432

    Article  Google Scholar 

  16. Sasanami T, Murata T, Ohtsuki M, Matsushima K, Hiyama G, Kansaku N, Mori M (2007) Induction of sperm acrosome reaction by perivilelline membrane glycoprotein ZP1 in Japanese quail (Coturnix japonica). Reproduction 133:41–49

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Faculty of Science, Hokkaido University, Kita 10 Nishi 8, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan

    Shusei Mizushima

  2. Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka, 422-8529, Japan

    Mei Matsuzaki & Tomohiro Sasanami

  3. United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Mei Matsuzaki & Tomohiro Sasanami

Authors
  1. Shusei Mizushima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mei Matsuzaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tomohiro Sasanami
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tomohiro Sasanami .

Editor information

Editors and Affiliations

  1. IRCMS, Kumamoto University, Kumamoto, Kumamoto, Japan

    Guojun Sheng

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Mizushima, S., Matsuzaki, M., Sasanami, T. (2017). Handling of Gametes for In Vitro Insemination in Birds. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7216-6_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7215-9

  • Online ISBN: 978-1-4939-7216-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation