Abstract
In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.
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Acknowledgments
This work was supported by grants from INRA and from ANR with the CRB-ANIM project (CRB-ANIM-ANR-11-INBS-0003).
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Fuet, A., Pain, B. (2017). Chicken Induced Pluripotent Stem Cells: Establishment and Characterization. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_14
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DOI: https://doi.org/10.1007/978-1-4939-7216-6_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7215-9
Online ISBN: 978-1-4939-7216-6
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