Abstract
Directed differentiation is a powerful cell culture technique where developmental pathways are applied to a pluripotent progenitor in order to generate specific terminally differentiated cell populations. Here, we describe a serum-free protocol using growth factors in defined concentrations to derive iPSC-hepatic cells starting from both feeder and feeder-free conditions. The generated iPSC-hepatic cells are developmentally similar to fetal stage hepatocytes, and when generated from patients with genetic mutations such as alpha-1 antitrypsin deficiency recapitulate pathologic changes associated with clinical disease, such as protein misfolding, intracellular retention of misfolded proteins, and elevated levels of ER stress.
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Acknowledgments
This work was supported by NIH/NIDDK grant R01DK101501 and the Alpha-1 Foundation.
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Kaserman, J.E., Wilson, A.A. (2017). Protocol for Directed Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) to a Hepatic Lineage. In: Borel, F., Mueller, C. (eds) Alpha-1 Antitrypsin Deficiency . Methods in Molecular Biology, vol 1639. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7163-3_15
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DOI: https://doi.org/10.1007/978-1-4939-7163-3_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7161-9
Online ISBN: 978-1-4939-7163-3
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