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Measurement of Oncometabolites d-2-Hydroxyglutaric Acid and l-2-Hydroxyglutaric Acid

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Acute Myeloid Leukemia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1633))

Abstract

We describe a liquid chromatography-tandem mass spectrometry assay for measurement of d-2-hydroxyglutaric acid and l-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of d-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.

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Author information

Authors and Affiliations

  1. Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Patricia M. Jones & Dinesh Rakheja M.D.

  2. Department of Pathology and Laboratory Medicine, Children’s Health, Children’s Medical Center, Dallas, TX, USA

    Patricia M. Jones, Richard Boriack & Dinesh Rakheja M.D.

  3. Metabolic Unit, Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands

    Eduard A. Struys

  4. Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Dinesh Rakheja M.D.

Authors
  1. Patricia M. Jones
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  2. Richard Boriack
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  3. Eduard A. Struys
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  4. Dinesh Rakheja M.D.
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Corresponding author

Correspondence to Dinesh Rakheja M.D. .

Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Sidney Kimmel Cancer Center, Philadelphia, Pennsylvania, USA

    Paolo Fortina

  2. Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Eric Londin

  3. Department of Pathology, UT Southwestern Medical Center and Children’s Medical Center, Dallas, Texas, USA

    Jason Y. Park

  4. Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA

    Larry J. Kricka

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Jones, P.M., Boriack, R., Struys, E.A., Rakheja, D. (2017). Measurement of Oncometabolites d-2-Hydroxyglutaric Acid and l-2-Hydroxyglutaric Acid. In: Fortina, P., Londin, E., Park, J., Kricka, L. (eds) Acute Myeloid Leukemia. Methods in Molecular Biology, vol 1633. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7142-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7142-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7140-4

  • Online ISBN: 978-1-4939-7142-8

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