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A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation

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Acute Myeloid Leukemia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1633))

Abstract

The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model.

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Acknowledgments

Zebrafish research was supported by Faculty Core Zebrafish Facility, LKS Faculty of Medicine, HKU. This work was supported by HKU771613M, HKUST5/CRF/12R, CityU9/CRF/13G, HMRF02132326, HMRF03143756 and HKU Seed Funding for Basic Research (201401159004, 201411159098). AYHL is the Li Shu Fan Medical Foundation Professor in Haematology and received funding from its endowment.

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Authors and Affiliations

  1. Division of Haematology, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong

    Alvin C. H. Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo & Anskar Y. H. Leung M.D.

Authors
  1. Alvin C. H. Ma
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  2. Xiangguo Shi
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  3. Bai-Liang He
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  4. Yuhan Guo
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  5. Anskar Y. H. Leung M.D.
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Corresponding author

Correspondence to Anskar Y. H. Leung M.D. .

Editor information

Editors and Affiliations

  1. Thomas Jefferson University, Sidney Kimmel Cancer Center, Philadelphia, Pennsylvania, USA

    Paolo Fortina

  2. Department of Pathology, Anatomy and Cell Biology, Sidney Kimmel Medical College Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Eric Londin

  3. Department of Pathology, UT Southwestern Medical Center and Children’s Medical Center, Dallas, Texas, USA

    Jason Y. Park

  4. Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA

    Larry J. Kricka

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Ma, A.C.H., Shi, X., He, BL., Guo, Y., Leung, A.Y.H. (2017). A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation. In: Fortina, P., Londin, E., Park, J., Kricka, L. (eds) Acute Myeloid Leukemia. Methods in Molecular Biology, vol 1633. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7142-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7142-8_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7140-4

  • Online ISBN: 978-1-4939-7142-8

  • eBook Packages: Springer Protocols

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