Abstract
In order to understand plant stress tolerance and its application, it is important to identify the signaling components involved in the stress-regulated gene expression. One initial step for this is generation of a stress-inducible luminescent Arabidopsis and its use in genetic mutant screening. Here, we describe how to generate a transgenic Arabidopsis line harboring a single copy of the STABILIZED1 (STA1) promoter-driven luciferase transgene (STA1p-LUC) as an example. STA1 is a pre-mRNA splicing factor Prp6p homolog and is induced by cold and heat stresses. In addition, generation of the STA1p-LUC mutant pool and a luminescence imaging-based screening for STA1p-LUC deregulated mutants are described.
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Acknowledgment
The work was supported by Next-Generation BioGreen21 Program (PJ011006), Rural Development Administration, Republic of Korea and the National Research Foundation of Korea (NRF) grant (2014R1A1A2058769) funded by the Ministry of Education, Republic of Korea.
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Yu, Si., Lee, Bh. (2017). Generation of a Stress-Inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-Responsive Gene Deregulation Mutants. In: Sunkar, R. (eds) Plant Stress Tolerance. Methods in Molecular Biology, vol 1631. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7136-7_6
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DOI: https://doi.org/10.1007/978-1-4939-7136-7_6
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7136-7
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