Abstract
Measuring rheological properties of cells in monolayers enables quantifying average cell properties in single experimental runs despite large cell-to-cell variations. Here, we describe how to modify a commercial rotational rheometer to accomplish the necessary precision for a monolayer rheometer and we delineate the steps for setting up experiments detecting average viscoelastic cell properties.
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References
Chhabra RP, Richardson JF (2008) Non-Newtonian flow and applied rheology, 2nd edn. Butterworth-Heinemann, Oxford, pp 1–55
Steffe JF (1996) Rheological methods in food process engineering. Freeman Press, East Lansing
Verdier C, Etienne J, Duperray A et al (2009) Review: rheological properties of biological materials. Comptes Rendus Physique 10:790–811
Kollmannsberger P, Fabry B (2011) Linear and nonlinear rheology of living cells. Annu Rev Mater Res 41:75–97
Weissman-Shomer P, Fry M (1975) Chick embryo fibroblasts senescence in vitro: Pattern of cell division and life span as a function of cell density. Mech Ageing Dev 4:159–166
Cai P, Mizutani Y, Tsuchiya M et al (2013) Quantifying cell-to-cell variation in power-law rheology. Biophys J 105:1093–1102
Fernández P, Heymann L, Ott A et al (2007) Shear rheology of a cell monolayer. New J Phys 9:419–447
Davies GA, Stokes JR (2005) On the gap error in parallel plate rheometry that arises from the presence of air when zeroing the gap. J Rheol 49:919–922
Davies GA, Stokes JR (2008) Thin film and high shear rheology of multiphase complex fluids. J Non-Newtonian Fluid Mech 148:73–87
Pipe CJ, Majmudar TS, Mckinley GH (2008) High shear rate viscometry. Rheol Acta 47:621–642
Dakhil H, Gilbert DF, Malhotra D et al (2016) Measuring average rheological quantities of cell monolayers in the linear viscoelastic regime. Rheol Acta 55:527–536
Dakhil H, Wierschem A (2014) Measuring low viscosities and high shear rates with a rotational rheometer in a thin-gap parallel-disk configuration. Appl Rheol 24:63795–63801
Gilbert DF, Meinhof T, Pepperkok R et al (2009) DetecTiff©: a novel image analysis routine for high-content screening microscopy. J Biomol Screen 14:944–955
Acknowledgments
We gratefully acknowledge financial support by Deutscher Akademischer Austauschdienst (DAAD), Germany, and the Ministry of Higher Education & Scientific Research, Iraq.
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Dakhil, H., Wierschem, A. (2017). Average Rheological Quantities of Cells in Monolayers. In: Gilbert, D., Friedrich, O. (eds) Cell Viability Assays. Methods in Molecular Biology, vol 1601. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6960-9_20
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DOI: https://doi.org/10.1007/978-1-4939-6960-9_20
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6960-9
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