Abstract
The method described here enables the automatic stretching and patterning of single DNA molecules onto a solid surface. It does not require chemical modification of the DNA or surface modification of the substrate. To detect a signal variation caused by sequence-specific dye binding or partial melting, it is crucial that the DNA molecules are arrayed in a parallel direction inside the narrow microscopic field. The method uses zigzag-shaped microgrooves in a densely-arranged molecular patterning apparatus in a microfluidic channel. By syringing through the microchannel, over 1500 DNA molecules can be arrayed simultaneously in the microgrooves. It will therefore serve as a template preparation for DNA molecular diagnosis by high-resolution imaging.
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Acknowledgment
This work was supported by JSPS KAKENHI Grant Number 26750146. This research is partly supported by the Japan Society for the Center of Innovation Program from Japan Science and Technology Agency, JST.
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Onoshima, D., Baba, Y. (2017). Microfluidic DNA Stretching Device for Single-Molecule Diagnostics. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_8
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DOI: https://doi.org/10.1007/978-1-4939-6734-6_8
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