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Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars

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Microchip Diagnostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1547))

Abstract

Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.

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Acknowledgment

This work was partially supported by the Japan Society for the Promotion of Science (JSPS, grant no. 24245013). We would like to thank Kansai Paint Co., Ltd., for valuable discussions.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Toshihiro Kasama, Noritada Kaji, Manabu Tokeshi & Yoshinobu Baba

  2. ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Noritada Kaji

  3. Division of Biotechnology and Macromolecular Chemistry, Hokkaido University, Sapporo, Japan

    Manabu Tokeshi & Yoshinobu Baba

  4. Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Yoshinobu Baba

  5. Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, 761-0395, Japan

    Yoshinobu Baba

  6. ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan

    Yoshinobu Baba

Authors
  1. Toshihiro Kasama
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  2. Noritada Kaji
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  3. Manabu Tokeshi
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  4. Yoshinobu Baba
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Corresponding author

Correspondence to Yoshinobu Baba .

Editor information

Editors and Affiliations

  1. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, Paris, France

    Valérie Taly

  2. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Jean-Louis Viovy

  3. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Stéphanie Descroix

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Kasama, T., Kaji, N., Tokeshi, M., Baba, Y. (2017). Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6734-6_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6732-2

  • Online ISBN: 978-1-4939-6734-6

  • eBook Packages: Springer Protocols

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