Skip to main content
SpringerLink
Log in

Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists

  • Protocol
  • First Online:
Microchip Diagnostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1547))

Abstract

The miniaturization of immunoassays using microfluidic devices is attractive for many applications, but an important challenge remains the patterning of capture antibodies (cAbs) on the surface of microfluidic structures. Here, we describe how to pattern cAbs on planar poly(dimethylsiloxane) (PDMS) stamps and how to microcontact print the cAbs on a dry-film resist (DFR). DFRs are new types of photoresists having excellent chemical resistance and good mechanical, adhesive, and optical properties. Instead of being liquid photoresists, DFRs are thin layers that are easy to handle, cut, photo-pattern, and laminate over surfaces. We show how to perform a simple fluorescence immunoassay using anti-biotin cAbs patterned on a 50-μm-thick DF-1050 DFR, Atto 647N-biotin analytes, and capillary-driven chips fabricated in silicon.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 119.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Bange A, Halsall HB, Heineman WR (2005) Microfluidic immunosensor systems. Biosens Bioelectron 20:2488–2503

    Article  CAS  Google Scholar 

  2. Auroux PA, Iossifidis D, Reyes DR et al (2002) Micro total analysis systems. 2. Analyticalstandard operations and applications. Anal Chem 74:2637–2652

    Article  CAS  Google Scholar 

  3. Temiz Y, Lovchik RD, Kaigala GV et al (2015) Lab-on-a-chip devices: how to close and plug the lab? Microelectron Eng 132:156–175

    Article  CAS  Google Scholar 

  4. Hitzbleck M, Delamarche E (2013) Reagents in microfluidics: an ‘in’ and ‘out’ challenge. Chem Soc Rev 42:8494–8516

    Article  CAS  Google Scholar 

  5. Nakanishi K, Sakiyama T, Imamura K (2001) On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon. J Biosci Bioeng 91:233–244

    Article  CAS  Google Scholar 

  6. Hawkes R, Niday E, Gordon J (1982) A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 119:142–147

    Article  CAS  Google Scholar 

  7. Ziegler J, Zimmermann M, Hunziker P et al (2008) High-performance immunoassays based on through-stencil patterned antibodies and capillary systems. Anal Chem 80:1763–1769

    Article  CAS  Google Scholar 

  8. Foley J, Schmid H, Stutz R et al (2005) Microcontact printing of proteins inside microstructures. Langmuir 21:11296–11303

    Article  CAS  Google Scholar 

  9. Bernard A, Michel B, Delamarche E (2001) Micromosaic immunoassays. Anal Chem 73:8–12

    Article  CAS  Google Scholar 

  10. Fan R, Vermesh O, Srivastava A et al (2008) Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood. Nat Biotechnol 26:1373–1378

    Article  CAS  Google Scholar 

  11. Niemeyer CM, Sano T, Smith CL et al (1994) Oligonucleotide directed self-assembly of proteins: semisynthetic DNA-streptavidin hybrid molecules as connectors for the generation of macroscopic arrays and the construction of supramolecular bioconjugates. Nucleic Acids Res 22:5530

    Article  CAS  Google Scholar 

  12. Yatsushiro S, Akamine R, Yamamura S et al (2011) Quantitative analysis of serum procollagen type I C-terminal propeptide by immunoassay on microchip. PLoS One 6:e18807

    Article  CAS  Google Scholar 

  13. Kumar A, Whitesides GM (1993) Features of gold having micrometer to centimeter dimensions can be formed through a combination of stamping with an elastomeric stamp and an alkanethiol “ink” followed by chemical etching. Appl Phys Lett 63:2002–2004

    Article  CAS  Google Scholar 

  14. Xia Y, Whitesides GM (1998) Soft lithography. Angew Chem Int Ed 37:550–575

    Article  CAS  Google Scholar 

  15. Bernard A, Delamarche E, Schmid H et al (1998) Printing patterns of proteins. Langmuir 14:2225–2229

    Article  CAS  Google Scholar 

  16. Bernard A, Renault JP, Michel B et al (2000) Microcontact printing of proteins. Adv Mater 12:1067–1070

    Article  CAS  Google Scholar 

  17. Tan JL, Tien J, Chen CS (2001) Microcontact printing of proteins on mixed self-assembled monolayers. Langmuir 18:519–523

    Article  Google Scholar 

  18. Coyer SR, García AJ, Delamarche E (2007) Facile preparation of complex protein architectures with sub-100-nm resolution on surfaces. Angew Chem Int Ed 46:6837–6840

    Article  CAS  Google Scholar 

  19. Vulto P, Glade N, Altomare L et al (2005) Microfluidic channel fabrication in dry film resist for production and prototyping of hybrid chips. Lab Chip 5:158–162

    Article  CAS  Google Scholar 

  20. Temiz Y, Delamarche E (2014) ‘Chip-olate’ and dry-film resists for efficient fabrication, singulation and sealing of microfluidic chips. J Micromech Microeng 24:097001

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. IBM Research GmbH, Saumerstrasse 4, 8803, Rüschlikon, Switzerland

    Yuksel Temiz, Robert D. Lovchik & Emmanuel Delamarche

Authors
  1. Yuksel Temiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert D. Lovchik
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Emmanuel Delamarche
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Emmanuel Delamarche .

Editor information

Editors and Affiliations

  1. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, Paris, France

    Valérie Taly

  2. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Jean-Louis Viovy

  3. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Stéphanie Descroix

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Temiz, Y., Lovchik, R.D., Delamarche, E. (2017). Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6734-6_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6732-2

  • Online ISBN: 978-1-4939-6734-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation