Abstract
Relevant animal models for Campylobacter jejuni infection have been difficult to establish due to C. jejuni’s inability to cause disease in many common animal research models. Fortunately, recent work has proven successful in developing several new and relevant mouse models of C. jejuni infection, including the SIGIRR-deficient mouse strain that develops acute enterocolitis in response to C. jejuni. Here we describe how to properly infect mice with C. jejuni, as well as a number of accompanying histological techniques to aid in studying C. jejuni colonization and infection in mice.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Newell DG (2001) Animal models of Campylobacter jejuni colonization and disease and the lessons to be learned from similar Helicobacter pylori models. Symp Ser 30:57S–67S
Babakhani FK, Bradley GA, Joens LA (1993) Newborn piglet model for campylobacteriosis. Infect Immun 61(8):3466–3475
Fox JG, Ackerman JI, Taylor N et al (1987) Campylobacter jejuni infection in the ferret: an animal model of human campylobacteriosis. Am J Vet Res 48(1):85–90
Bereswill S, Fischer A, Plickert R et al (2011) Novel murine infection models provide deep insights into the “menage a trois” of Campylobacter jejuni, microbiota and host innate immunity. PLoS One 6(6):e20953. doi:10.1371/journal.pone.0020953
Mansfield LS, Bell JA, Wilson DL et al (2007) C57BL/6 and congenic interleukin-10-deficient mice can serve as models of Campylobacter jejuni colonization and enteritis. Infect Immun 75(3):1099–1115. doi:10.1128/IAI.00833-06
Stahl M, Ries J, Vermeulen J et al (2014) A novel mouse model of Campylobacter jejuni gastroenteritis reveals key pro-inflammatory and tissue protective roles for Toll-like receptor signaling during infection. PLoS Pathog 10(7):e1004264. doi:10.1371/journal.ppat.1004264
Chang C, Miller JF (2006) Campylobacter jejuni colonization of mice with limited enteric flora. Infect Immun 74(9):5261–5271. doi:10.1128/IAI.01094-05
Lippert E, Karrasch T, Sun X et al (2009) Gnotobiotic IL-10; NF-kappaB mice develop rapid and severe colitis following Campylobacter jejuni infection. PLoS One 4(10):e7413. doi:10.1371/journal.pone.0007413
Sham HP, Yu EY, Gulen MF et al (2013) SIGIRR, a negative regulator of TLR/IL-1R signalling promotes Microbiota dependent resistance to colonization by enteric bacterial pathogens. PLoS Pathog 9(8):e1003539. doi:10.1371/journal.ppat.1003539
Khan MA, Steiner TS, Sham HP et al (2010) The single IgG IL-1-related receptor controls TLR responses in differentiated human intestinal epithelial cells. J Immunol 184(5):2305–2313. doi:10.4049/jimmunol.0900021
Mahler M, Bristol IJ, Leiter EH et al (1998) Differential susceptibility of inbred mouse strains to dextran sulfate sodium-induced colitis. Am J Physiol 274(3 Pt 1):G544–G551
Strachan NJ, Watson RO, Novik V et al (2008) Sexual dimorphism in campylobacteriosis. Epidemiol Infect 136(11):1492–1495. doi:10.1017/S0950268807009934
Rogers GB, Kozlowska J, Keeble J et al (2014) Functional divergence in gastrointestinal microbiota in physically-separated genetically identical mice. Sci Rep 4:5437. doi:10.1038/srep05437
Thoene-Reineke C, Fischer A, Friese C et al (2014) Composition of intestinal microbiota in immune-deficient mice kept in three different housing conditions. PLoS One 9(11):e113406. doi:10.1371/journal.pone.0113406
Watson RO, Novik V, Hofreuter D et al (2007) A MyD88-deficient mouse model reveals a role for Nramp1 in Campylobacter jejuni infection. Infect Immun 75(4):1994–2003. doi:10.1128/IAI.01216-06
McAuley JL, Linden SK, Png CW et al (2007) MUC1 cell surface mucin is a critical element of the mucosal barrier to infection. J Clin Invest 117(8):2313–2324. doi:10.1172/JCI26705
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Stahl, M., Graef, F.A., Vallance, B.A. (2017). Mouse Models for Campylobacter jejuni Colonization and Infection. In: Butcher, J., Stintzi, A. (eds) Campylobacter jejuni. Methods in Molecular Biology, vol 1512. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6536-6_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6536-6_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6534-2
Online ISBN: 978-1-4939-6536-6
eBook Packages: Springer Protocols