Abstract
Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purification of meiotic and post-meiotic male germ cells from adult mouse testes. We also describe how the fractionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming.
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Acknowledgment
This work is supported by a grant from “Foundation pour la Recherche Medicale (FRM)” “analyse bio-informatique pour la recherche en biologie” program as well as by ANR EpiSperm2 and Episperm3 programs and by INCA libre program.
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Buchou, T. et al. (2017). Purification and Analysis of Male Germ Cells from Adult Mouse Testis. In: Krämer, O. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 1510. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6527-4_12
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DOI: https://doi.org/10.1007/978-1-4939-6527-4_12
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6525-0
Online ISBN: 978-1-4939-6527-4
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