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In Vivo Biosensor Based on Prussian Blue for Brain Chemistry Monitoring : Methodological Review and Biological Applications

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In Vivo Neuropharmacology and Neurophysiology

Part of the book series: Neuromethods ((NM,volume 121))

Abstract

The scope of the present chapter is to give a brief outline of how Prussian Blue (PB) may be used in biosensor applications, especially in neuroscience, and how to mitigate its main limitation (its instability at physiological pH). During the last decade, a large number of studies involving PB have appeared exploring different biosensor configurations (carbon paste, screen printing, glassy carbon, etc.) and different oxidoreductase enzymes (e.g., glucose oxidase, lactate oxidase, and glutamate oxidase). Nevertheless, its applications in neuroscience were not described until 2010 by our group. Later, several publications developing glucose and lactate microbiosensors, based on PB-modified carbon fiber electrodes, were reported. In addition, the use of several cationic surfactants such as cetyltrimethylammonium bromide (CTAB), benzethonium chloride (BZT), and cetylpyridinium chloride (CPC), during electrochemical deposition of PB, demonstrated the ability of this hybrid configuration to improve the pH stability and the electrochemical performance of PB films for in-vivo biosensing applications.

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Acknowledgments

This research was supported by the grant INNPACTO-MINECO (IPT-2012-0961-300000), Ministerio de Ciencia e Innovación (TIN2011-28146), project RECUPERA 2020 from MINECO and the “Fondo social Europeo”, project MAT2013-40852-R and MAT2013-42900-P from MINECO and TEP 8067 and FQM 6900 from the Junta de Andalucía.

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Authors and Affiliations

  1. Section of Physiology, Department of Basic Medical Sciences, School of Health Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain

    Pedro Salazar & José Luis González-Mora

  2. Neurochemistry and Neuroimaging Group, Laboratory of Sensors, Biosensors and Materials, Faculty of Medical Sciences, University of La Laguna, Campus de Ofra s/n, 38071, La Laguna, Tenerife, Spain

    Pedro Salazar, Miriam Martín & José Luis González-Mora

  3. UCD School of Chemistry, University College Dublin, Belfield, Dublin, D04 N2E5, Ireland

    Robert D. O’Neill

Authors
  1. Pedro Salazar
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  2. Miriam Martín
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  3. Robert D. O’Neill
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  4. José Luis González-Mora
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Corresponding author

Correspondence to Pedro Salazar .

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Editors and Affiliations

  1. Department of Pharmacology and Toxicology, University of Innsbruck, Innsbruck, Austria

    Athineos Philippu

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Salazar, P., Martín, M., O’Neill, R.D., González-Mora, J.L. (2017). In Vivo Biosensor Based on Prussian Blue for Brain Chemistry Monitoring : Methodological Review and Biological Applications. In: Philippu, A. (eds) In Vivo Neuropharmacology and Neurophysiology. Neuromethods, vol 121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6490-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6490-1_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6488-8

  • Online ISBN: 978-1-4939-6490-1

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