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Optogenetic Manipulations of Neuronal Network Oscillations: Combination of Optogenetics and Electrophysiological Recordings in Behaving Mice

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In Vivo Neuropharmacology and Neurophysiology

Part of the book series: Neuromethods ((NM,volume 121))

Abstract

Neuronal network oscillations support interaction within and between brain regions and are implicated in cognitive functions including perception, memory, and spatial navigation. Recent developments of optogenetics and in vivo elecrophysiology made possible a high-precision control of network oscillations. Here, we describe an experimental approach established in our laboratory to optogenetically manipulate hippocampal theta (5–10 Hz) oscillations in behaving mice and thus to causally address their role in behavior. We provide detailed description of this preparation, describe its advantages and limitations and discuss further possible applications.

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Acknowledgements

We thank Franziska Bender, Maria Gorbati, Marta Carus, Xiaojie Gao, and Suzanne van der Veldt for their valuable contributions to the results and protocols described here. This work was supported by the Deutsche Forschungsgemeinschaft (DFG; Exc 257 NeuroCure, TK and AP; SPP1665, AP), The Human Frontier Science Program (HFSP; RGY0076/2012, TK), and The German-Israeli Foundation for Scientific Research and Development (GIF; I-1326-421.13/2015, TK).

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Authors and Affiliations

  1. Behavioral Neurodynamics Group, Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Street 10, Berlin, 13125, Germany

    Tatiana Korotkova & Alexey Ponomarenko

  2. NeuroCure Cluster of Excellence, Charite Universitätsmedizin, Chariteplatz 1, Virchowweg 6, CCO, Berlin, 10117, Germany

    Tatiana Korotkova & Alexey Ponomarenko

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  1. Tatiana Korotkova
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  2. Alexey Ponomarenko
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Correspondence to Tatiana Korotkova or Alexey Ponomarenko .

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  1. Department of Pharmacology and Toxicology, University of Innsbruck, Innsbruck, Austria

    Athineos Philippu

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Korotkova, T., Ponomarenko, A. (2017). Optogenetic Manipulations of Neuronal Network Oscillations: Combination of Optogenetics and Electrophysiological Recordings in Behaving Mice. In: Philippu, A. (eds) In Vivo Neuropharmacology and Neurophysiology. Neuromethods, vol 121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6490-1_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6490-1_4

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