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The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information

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Plant Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1482))

Abstract

Conservation of particular molecular sequence motifs throughout evolution is a strong indicator of their functional relevance as selective pressure likely prevented the accumulation of mutations. Known as “phylogenetic footprinting”, this rationale has been exploited for the identification of novel functional motifs using sequence information from sequence alignments of diverse species, in particular transcription factor binding site motifs in aligned gene promoter sequences of orthologous genes. With the rapid advances of sequencing technologies, whole genome sequence information is accumulating not only across different species, but increasingly for variants of the same species exhibiting relatively little sequence variability, primarily present as single nucleotide polymorphisms (SNPs). Here, we lay out the basic strategy for the identification of functional cis-regulatory motifs in gene promoter regions based on SNP information.

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Abbreviations

TFBS:

Transcription factor binding site

TSS:

Transcription start site

SNP:

Single nucleotide polymorphism

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Author information

Authors and Affiliations

  1. Max Planck Institute for Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany

    Paula Korkuć & Dirk Walther

Authors
  1. Paula Korkuć
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  2. Dirk Walther
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Corresponding author

Correspondence to Dirk Walther .

Editor information

Editors and Affiliations

  1. Institut für Genetik, Technische Universität Braunschweig, Braunschweig, Germany

    Reinhard Hehl

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Korkuć, P., Walther, D. (2016). The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information. In: Hehl, R. (eds) Plant Synthetic Promoters. Methods in Molecular Biology, vol 1482. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6396-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6396-6_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6394-2

  • Online ISBN: 978-1-4939-6396-6

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