Abstract
Transcription factors often form protein complexes and give rise to intricate transcriptional networks. The regulation of transcription factor multimerization plays a key role in the fine-tuning of the underlying transcriptional pathways and can be exploited to modulate synthetic transcriptional modules. A novel regulation of protein complex formation is emerging: microProteins—truncated transcription factors—engage in protein–protein interactions with transcriptional complexes and modulate their transcriptional activity. Here, we outline a strategy for the discovery, design, and test of putative miPs to fine-tune the activity of transcription factors regulating synthetic or natural transcriptional circuits.
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Fiume, E., de Klein, N., Rhee, S.Y., Magnani, E. (2016). A Framework for Discovering, Designing, and Testing MicroProteins to Regulate Synthetic Transcriptional Modules. In: Hehl, R. (eds) Plant Synthetic Promoters. Methods in Molecular Biology, vol 1482. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6396-6_12
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DOI: https://doi.org/10.1007/978-1-4939-6396-6_12
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