Abstract
Islet transplantation (IT) has recently been shown to be a promising alternative to pancreas transplantation for reversing diabetes. IT requires the isolation of the islets from the pancreas, and these islets can be used to fabricate a bio-artificial pancreas. Enzymatic digestion is the current gold standard procedure for islet isolation but has lingering concerns. One such concern is that it has been shown to damage the islets due to nonselective tissue digestion. This chapter provides a detailed description of a nonenzymatic method that we are exploring in our lab as an alternative to current enzymatic digestion procedures for islet isolation from human and nonhuman pancreatic tissues. This method is based on selective destruction and protection of specific cell types and has been shown to leave the extracellular matrix (ECM) of islets intact, which may thus enhance islet viability and functionality. We also show that these SOS-isolated islets can be microencapsulated for transplantation.
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References
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Disclosure
Giuseppe Orlando is member of the BioCaPan Consortium which has received funding from the European Union’s Horizon2020 research and innovation program under grant agreement No 646272. Kevin Enck and Riccardo Tamburrini are funded through BioCaPan.
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Enck, K., McQuilling, J.P., Orlando, G., Tamburrini, R., Sivanandane, S., Opara, E.C. (2017). Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation. In: Opara, E. (eds) Cell Microencapsulation. Methods in Molecular Biology, vol 1479. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6364-5_14
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DOI: https://doi.org/10.1007/978-1-4939-6364-5_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6362-1
Online ISBN: 978-1-4939-6364-5
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