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Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes

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Non-Viral Gene Delivery Vectors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1445))

Abstract

Niosome formulations for gene delivery purposes are based on nonionic surfactants, helper lipids, and cationic lipids that interact electrostatically with negatively charged DNA molecules to form the so-called nioplexes. Niosomes are elaborated by different techniques, such as solvent emulsion-evaporation, thin film hydration, hand-shaking, dissolvent injection, and microfluidization method, among many others. In this chapter, we have described some protocols for the elaboration of niosomes and nioplexes and their physicochemical characterization that guarantees the quality criteria of the formulation in terms of size, morphology, ΞΆ-potential, and stability.

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Acknowledgments

This project was partially supported by the University of the Basque Country UPV/EHU (UFI 11/32), the National Council of Science and Technology (CONACYT), Mexico, Reg. # 217101, the Spanish Ministry of Education (Grant CTQ2010-20541, CTQ2010-14897), the Basque Government (Department of Education, University and Research, pre-doctoral PRE-2014-1-433 and BFI-2011-2226 grants) and by Spanish grants MAT2012-39290-C02-01 and IPT-2012-0574-300000. Technical and human support provided by SGIker (UPV/EHU) is gratefully acknowledged. Authors also wish to thank the intellectual and technical assistance from the ICTS β€œNANBIOSIS”, more specifically by the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the University of Basque Country (UPV/EHU).

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Authors and Affiliations

  1. NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain

    Edilberto Ojeda,Β Mireia Agirre,Β Ilia Villate-Beitia,Β Mohamed Mashal,Β Gustavo Puras,Β Jon ZarateΒ &Β Jose L. Pedraz

  2. Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain

    Edilberto Ojeda,Β Mireia Agirre,Β Ilia Villate-Beitia,Β Gustavo Puras,Β Jon ZarateΒ &Β Jose L. Pedraz

  3. Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country, 01006, Vitoria-Gasteiz, Spain

    Jose L. Pedraz

Authors
  1. Edilberto Ojeda
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  2. Mireia Agirre
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  3. Ilia Villate-Beitia
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  4. Mohamed Mashal
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  5. Gustavo Puras
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  6. Jon Zarate
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  7. Jose L. Pedraz
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Corresponding author

Correspondence to Jose L. Pedraz .

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Editors and Affiliations

  1. Polytechnic University of Milan, Milan, Italy

    Gabriele Candiani

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Β© 2016 Springer Science+Business Media New York

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Ojeda, E. et al. (2016). Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes. In: Candiani, G. (eds) Non-Viral Gene Delivery Vectors. Methods in Molecular Biology, vol 1445. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3718-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3718-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3716-5

  • Online ISBN: 978-1-4939-3718-9

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