Abstract
Histone deacetylases (HDACs) have a central role in the regulation of gene expression, which undergoes alternative splicing during embryonic stem cell (ES) cell differentiation. Alternative splicing gives rise to vast diversity over gene information, arousing public concerns in the last decade. In this chapter, we describe a strategy to detect HDAC7 alternative splicing and analyze its function on ES cell differentiation.
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Acknowledgement
This study was funded by British Heart Foundation project grant PG13-63-30419.
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Yang, J., Margariti, A., Zeng, L. (2016). Analysis of Histone Deacetylase 7 (HDAC7) Alternative Splicing and Its Role in Embryonic Stem Cell Differentiation Toward Smooth Muscle Lineage. In: Sarkar, S. (eds) Histone Deacetylases. Methods in Molecular Biology, vol 1436. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3667-0_7
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DOI: https://doi.org/10.1007/978-1-4939-3667-0_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3665-6
Online ISBN: 978-1-4939-3667-0
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