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Sirtuin 6 (SIRT6) Activity Assays

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Histone Deacetylases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1436))

Abstract

SIRT6 has been shown to possess weak deacetylation, mono-ADP-ribosyltransferase activity, and deacylation activity in vitro. SIRT6 selectively deacetylates H3K9Ac and H3K56Ac. Several SIRT6 assays have been developed including HPLC assays, fluorogenic assays, FRET, magnetic beads, in silico, and bioaffinity chromatography assays. Herein, we describe detailed protocols for the HPLC based activity/inhibition assays, magnetic beads deacetylation assays, bioaffinity chromatographic assays as well as fluorogenic and in silico assays.

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Authors and Affiliations

  1. Bioanalytical and Drug Development Unit, National institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD, 21224-6825, USA

    Minna Rahnasto-Rilla & Ruin Moaddel

  2. School of Pharmacy, University of Eastern Finland, Kuopio, 70210, Finland

    Minna Rahnasto-Rilla & Maija Lahtela-Kakkonen

Authors
  1. Minna Rahnasto-Rilla
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  2. Maija Lahtela-Kakkonen
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  3. Ruin Moaddel
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Corresponding author

Correspondence to Ruin Moaddel .

Editor information

Editors and Affiliations

  1. Boston University School of Medicine, Boston, Massachusetts, USA

    Sibaji Sarkar

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© 2016 Springer Science+Business Media New York

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Rahnasto-Rilla, M., Lahtela-Kakkonen, M., Moaddel, R. (2016). Sirtuin 6 (SIRT6) Activity Assays. In: Sarkar, S. (eds) Histone Deacetylases. Methods in Molecular Biology, vol 1436. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3667-0_17

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  • DOI: https://doi.org/10.1007/978-1-4939-3667-0_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3665-6

  • Online ISBN: 978-1-4939-3667-0

  • eBook Packages: Springer Protocols

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