Abstract
Nicotiana benthamiana has shown great success as a platform for the production of recombinant proteins. Here, we describe methods to transiently express high levels of recombinant proteins and simultaneously modulate their glycosylation pattern toward human-like structures. The method aims to generate recombinant proteins with a targeted largely homogeneous glycosylation profile for structure–function studies.
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Acknowledgments
This work was supported by a grant from the Austrian Research Promotion Agency (FFG) Laura Bassi Centre of Expertise PlantBioP (Number 822757), and the Austrian Science Fund (FWF; Grant Number L575-B13).
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Castilho, A., Steinkellner, H. (2016). Transient Expression of Mammalian Genes in N. benthamiana to Modulate N-Glycosylation. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_7
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DOI: https://doi.org/10.1007/978-1-4939-3289-4_7
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