Abstract
Antibiotic-free, efficient in vitro selection in plant genetic engineering can improve risk perception and speed up pre-market scrutiny of genetically modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentially be applied, with minor adaptations, to many other monocot and dicot crop plants.
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Ferradini, N., Giancaspro, A., Nicolia, A., Gadaleta, A., Veronesi, F., Rosellini, D. (2016). Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_6
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DOI: https://doi.org/10.1007/978-1-4939-3289-4_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3288-7
Online ISBN: 978-1-4939-3289-4
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