Abstract
One of the major challenges in plant molecular biology is to generate transgenic plants that express transgenes stably over generations. Here, we describe some routine methods to study transgene locus structure and to analyze transgene expression in plants: Southern hybridization using DIG chemiluminescent technology for characterization of transgenic locus, SYBR Green-based real-time RT-PCR to measure transgene transcript level, and protein immunoblot analysis to evaluate accumulation and stability of transgenic protein product in the target tissue.
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Acknowledgements
The authors would like to thank Lina Torrizo, Marina Manzanilla, and Maria Gina Borja for help with rice transformation. Work at the GTL is supported by HarvestPlus.
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Trijatmiko, K.R., Arines, F.M., Oliva, N., Slamet-Loedin, I.H., Kohli, A. (2016). Molecular Analyses of Transgenic Plants. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_15
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DOI: https://doi.org/10.1007/978-1-4939-3289-4_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3288-7
Online ISBN: 978-1-4939-3289-4
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