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Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells

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Estrogen Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1366))

Abstract

Chromatin immunoprecipitation (ChIP) is a technique to investigate the interaction between proteins and DNA within the natural chromatin context of the cell. There are two previously identified two canonical estrogen response elements (ERE1 and ERE2) present in the 5′-flanking region of the Greb1 gene which is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the Greb1 promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the Greb1 promoter.

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Author information

Authors and Affiliations

  1. Department of OB/GYN & Women’s Health, University of Louisville School of Medicine, Louisville, KY, 40292, USA

    Jing Lin & Zhenmin Lei

  2. Department of OB/GYN & Women’s Health, University of Louisville Health Sciences Center, 412 A Building, 500 South Preston Street, Louisville, KY, 40202, USA

    Zhenmin Lei

Authors
  1. Jing Lin
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  2. Zhenmin Lei
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Corresponding author

Correspondence to Zhenmin Lei .

Editor information

Editors and Affiliations

  1. University of South Dakota Sanford School of Medicine, Vermillion, South Dakota, USA

    Kathleen M. Eyster

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Lin, J., Lei, Z. (2016). Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 1366. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3127-9_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3127-9_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3126-2

  • Online ISBN: 978-1-4939-3127-9

  • eBook Packages: Springer Protocols

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