Abstract
In situ hybridization (ISH) is an excellent method for detecting RNA in histological sections, both to detect gene expression and to assign gene expression to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of three estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) as well as the membrane-bound G-protein-coupled estrogen receptor 1 (GPER) in the human testis.
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Fietz, D., Bergmann, M., Hartmann, K. (2016). In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 1366. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3127-9_15
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DOI: https://doi.org/10.1007/978-1-4939-3127-9_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3126-2
Online ISBN: 978-1-4939-3127-9
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