Abstract
Understanding how genes are regulated, decoding their “regulome”, is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing the DNA-binding specificity of the vast majority of yeast transcription factors using Protein Binding Microarrays. This approach can be implemented to any given organism.
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Acknowledgment
We thank Shaheynoor Talukder for standard operating procedure and Timothy R. Hughes for data availability. We also thank Esther T. Chan for useful comments. G.B. work was supported by the CIHR, the Institut Pasteur and the Centre National pour la Recherche Scientifique. LPC’s work was supported by a NSERC Discovery Grant and Memorial University of Newfoundland.
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Peña-Castillo, L., Badis, G. (2016). Systematic Determination of Transcription Factor DNA-Binding Specificities in Yeast. In: Devaux, F. (eds) Yeast Functional Genomics. Methods in Molecular Biology, vol 1361. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3079-1_12
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DOI: https://doi.org/10.1007/978-1-4939-3079-1_12
Publisher Name: Humana Press, New York, NY
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