Abstract
Chromatin immunoprecipitation experiments are critical to investigating the interactions between DNA and a wide range of nuclear proteins within a cell or biological sample. In this chapter we outline an optimized protocol for genome-wide chromatin immunoprecipitation that has been used successfully for several distinct morphological forms of numerous yeast species, and include an optimized method for amplification of chromatin immunoprecipitated DNA samples and hybridization to a high-density oligonucleotide tiling microarray. We also provide detailed suggestions on how to analyze the complex data obtained from these experiments.
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Acknowledgments
This work was supported by National Institutes of Health (NIH) grants R00AI100896 and R01AI049187.
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Lohse, M.B., Kongsomboonvech, P., Madrigal, M., Hernday, A.D., Nobile, C.J. (2016). Genome-Wide Chromatin Immunoprecipitation in Candida albicans and Other Yeasts. In: Devaux, F. (eds) Yeast Functional Genomics. Methods in Molecular Biology, vol 1361. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3079-1_10
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DOI: https://doi.org/10.1007/978-1-4939-3079-1_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3078-4
Online ISBN: 978-1-4939-3079-1
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